Reversible oxidation of Azotobacter vinelandii polynucleotide phosphorylase
نویسندگان
چکیده
منابع مشابه
Preparatin, proteolysis and reversible oxidationof highly purified Azotobacter vinelandii polynucleotide phosphorylase.
1. A new method has been developed for the preparation in good yield of highly purified Azotobacter vinelandii polynucleotide phosphorylase in its reduced form. 2. Aging or digestion with trypsin causes the enzyme to develop a primer requirement that is not eliminated by beta-mercaptoethanol. 3. The development of a primer requirement is accompanied by marked changes of the electrophoretic mobi...
متن کاملThe terminal oxidation system of Azotobacter vinelandii.
Preliminary work showed that Azotobacter vinelandii strain 0 (A2otobacter agile var. vinelandii) yielded a more stable enzyme extract than any other species tried, and this organism was used throughout. Repaske (1954) and Alexander and Wilson (1954) have reported the essential details for growing and harvesting the culture and for making the enzyme preparations. The succinic dehydrogenase (SD) ...
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Benzoic acid metabolism was studied in Azotobacter vinelandii. 14 A radioactive intermediate of C-benzoic acid metabolism was discovered. Preliminary evidence suggests that this intermediate may be 2 ,-hydroxybenzoate but positive identification was not achieved, values for several hydroxybenzoates and catechol on paper chromato graphy in various solvent systems are given. The oxygenase, metap...
متن کاملPlasmids of Azotobacter vinelandii.
Four laboratory strains and two isolates of Azotobacter vinelandii were found to contain plasmids. Twenty-five laboratory strains which could fix nitrogen did not have free, covalently closed circular plasmid DNA. The plasmids varied in size from 9 to 52 megadaltons, and each strain yielded only one plasmid. No discernible differences in ability to fix nitrogen were found between plasmid-bearin...
متن کاملUltrastructure of Azotobacter vinelandii.
Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not reported previously: intine fibrils, intine vesicles, intine membrane, and mi...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1969
ISSN: 0306-3283
DOI: 10.1042/bj1120381